*MICROSCOPE, model Nikon Ti

 Type:  inverted 
 *Objective lenses:  CFI Plan Fluor 4x, 10x, 20x, 40x, 60x, CF Epi Plan APO 100x 
 Stage:  automated 
 – travel range:  114 x 75 mm 
 – accuracy (1 mm of translation) / XY repeatability:  0.06 μm / ± 1 μm 
 Z-scanner:  piezo scanner 
 – objective translation range:  80 μm 
 – minimal translation step / repeatability:  50 nm / < 6 nm 


 Optimized optics for the spectral range:  325 – 1100 nm 
 Laser radiation delivery:  single, double, triple or penta input port 
 Polarizers (excitation and detection channels):  Glan-Taylor prizm, 325 – 1000 nm 
 Half-wave plate (λ / 2) positioner:  five-position 
 Beam expander:  magnification factor 1 – 4 
 Edge filter positioner:  five-position 
 Interference filter positioner:  six-position 
 OMU and microscope coupling:  three- or five- position switch 
 Spatial resolution: XY: <300 nm, Z: 600 nm (532 nm laser, 100x, NA = 0.9)


 Optical configuration:  vertical 
 Focal length:  520 mm
 Ports:  1 input, 2 output 
 Flat field:  28 x 10 mm 
 Grating unit:  4-position turret 
 Grating choice:  150, 300, 600, 1200, 2400, 3600, l / mm, Echelle (75 l / mm) 
 Spectral resolution:  0.25 cm -1  Echelle grating, wavelength 500 nm)
 0.9 cm -1   (1800 I / mm grating) 
 Confocal pinhole:  width 0 – 1.5 mm; step size 0.5 μm 
 Wavelength accuracy with CCD camera:  0.005 nm  (1800 I / mm grating) 


 Scanning method:  galvanometer scanners of X – Y mirrors 
 Scanning speed:  3 sec (1001 x 1001 pixels, min step 20 nm) 
 Scanning region:  150 μm x 150 μm (using 100x objective lens) 


 Type:  digital CCD camera HS101H 
 Sensor:  back-thinned CCD array 2048 x 122 
 Pixel size:  12 x 12 μm 
 Cooling: Two-stage Peltier cooling with temperature stabilization to -45 °C 
 ADC:  16 bit 


 Objective positioner:  three-coordinated (X, Y, Z) 
 Laser beam attenuator:  VND filter 
 Confocal pinhole:  variable from 0 to 1.5 mm, step size 0.5 μm 
 Detector:  PMT 


 The system confi guration allows of using up to 5 lasers:          Power, mW 
 Type:    Wavelength, nm                
 HeCd laser:  325         10
 HeCd laser:  441.6      50 
 DPSS laser:  473         22 50
 DPSS laser:  532  22 50
 Helium-neon laser:  633  10 
 Semiconductor laser:  785  100 

* Microscope, objective lenses, and type of lasers can be offered on customer’s request



3D Scanning Laser Raman Microscope

Simultaneous / Multifunctional Analysis


  • Raman Measurements

  • Luminescence Measurements

  • Laser Reflection & Transmission Measurements  

  • Spectral and Polarization measurements


3D high-contrast images in reflected light

3D confocal Raman measurements

Confocal Detection Principle


Confocal Laser Scanning Raman Microscope has become a widely recognized research instrument in recent years. Confocal microscopy offers several advantages over conventional wide-field optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane and the capability to collect serial optical sections from thick samples. The image of the extended sample is generated by scanning the focused laser beam across a defined area.

The pinhole aperture rejects the residual scattered rays originated from any out-of-focus points on a sample. 

We have created the instrument that is right for you

High spectral resolution

Spatial resolution: less than 500 nm (Z), 200 nm (X, Y) 

Spectral resolution: ~ 0.25 cm-1 

Wavelength accuracy in spectrum with CCD detector: 0.005 nm (1800 l / mm)





High spatial resolution Raman confocal microscopy can provide information on dopant concentrations and stress distribution in semiconductor materials.


Raman spectroscopy allows easy visualization of cellular components with minimum perturbation.


Confocal Raman spectroscopy allows chemical compounds and molecular conformers in various drugs to be identified and their distribution mapped with high spatial resolution.


Confocal Raman microscopy is an excellent technique for characterization of minerals, detection of components distribution and their phase transitions.


Confocal Raman microspectroscopy is a promising technique which enables measuring the skin care products as well as their penetration capability.


Application areas include identification of unknown substances, different types of fibers, glasses, paints, explosive materials, inks, narcotic and toxic substances, proof of authenticity of documents.

Material science

Confocal Raman offers excellent spatial resolution for characterization of materials (superconductor, polymers, coatings, composites, carbon nanotubes, graphene, etc.).

Heritage and Art, Gemology


Raman spectroscopy allows identfication of pigments and binders used in paintings. The spectroscopic analysis of archaeological samples (ceramics, glasses, etc.) provides information on their origin and history. Raman technique allows rapid identfication of colored stones, natural and synthetic diamonds.


and many more…

Raman megapixel image for 3 sec

Fully automated system with up to 5 integrated lasers

High spatial resolution and sensitivity


Major features

The highest spectral and imaging resolution with specially designed spectrometer

Specially designed imaging spectrometer incorporates many features that make it ideal for confocal Raman measurements. The image of pinhole is projected to a multichannel detector without any aberrations.

The smaller amount of illuminated pixels on the CCD matrix leads to the smaller dark counts and the higher spectral resolution.

Spectral resolution of RAMOS N500 with an Echelle grating is 0.25 cm-1.

Spectral image of the pinhole on the CCD camera (aberration free).
CCD pixel size is 12 μm.

High optical throughput for enhanced sensitivity

The 4th order Silicon band at 1940 cm-1  can be observed in less than one minute using a low intensity laser.

2D / 3D images can be acquired rapidly.

Silicon 4th order sensitivity.

Fully automated


People with little or no experience in Raman spectroscopy can use RAMOS N500. The system is highly modular and fully automated. Up to 5 lasers can be used.

The lasers can be switched from one to another by just one click.

Motorized control for laser power, beam diameter, polarization orientation, pinhole size and grating is provided.


Low frequency Raman shift  measurements (down to 5 cm-1) with Bragg Super-Notch filters


True confocal design 

High spatial resolution

Laser Raman microscope RAMOS N500 can achieve:

  • lateral resolution close to theoretical limitation




 wavelength, nm 



XY – plane

 resolution, nm 

488 100x, NA = 0.9 250
532 100x, NA = 0.9 275
633 100x, NA = 0.9 320
785 100x, NA = 0.9 390

RAMOS N500 can take high definition Raman images (λ = 514 nm, 100x, NA = 1.4).


  • axial resolution (in depth direction, 100x, NA = 0.9)



Laser wavelength, nm 


Z (axial) resolution, nm 



Axial resolution of 450 nm (λ = 488 nm, 100x, NA = 0.95).

Wide Raman shift measurement range


Laser wavelength, nm 


 Wavenumber range, cm-1


325125 – 8000
355115 – 8000
47380 – 6000
53250 – 8000
63350 – 6000
78540 – 2800

Low-frequency Raman shift measurement range can be expanded using Bragg notch filters.

Low-frequency Raman bands of sulfur (lower than 250 cm1, 633 nm laser)

Megapixel Raman image for 3 sec

True confocal design 

High spatial resolution


3D scanning laser confocal Raman microscope RAMOS N500 provides the acquisition of two images within a single scan: a Rayleigh image (using laser light reflected from a sample) and a spectral image by Raman scattering.

Ultrafast imaging option allows to get confocal image in 3 sec (3 μs/pixel).

RAMOS N500 uses fast beam scanning by galvano mirrors.

Layout of galvano mirror scanner module allows mapping with no intensity losses from image center to its edges.


Rayleigh (1000 x 1000 pixels, time per 1 pixel is 3 μs) and Raman (1000 x 1000 pixels, time per 1 pixel is 43 μs) images of Granite Gneiss India. 
Anatase distribution.

Fast imaging mode with EMCCD / CCD

RAMOS N500 system can be used with a number of different detectors.

Up to three detectors can be used simultaneously. Proprietary algorithm for taking high speed of Raman imaging with fast spectral CCD (EMCCD) is offered.

The use of an EMCCD (Electron Multiplying CCD) camera can greatly increase Raman detection efficiency and speed.

Raman image of Silicon / SiOsample.
Si distribution (500 x 500 pixels, time per pixel is 5 ms).

Fully automated system Software package with powerful analytical functionality

Ultrawide field Raman imaging

Uniform, large size scanning area of a galvanic scanner module:

  • 150 μm x 150 μm (objective lens 100x)
  • 320 μm x 320 μm (objective lens 40x)
  • 680 μm x 680 μm (objective lens 20x)

Automatic XY stage can be used for ultra-wide field imaging.

The panoramic image (hyper image) by automatic stitching of a series of images obtained with the use of galvanic scanner.

High precision spectrometer calibration

RAMOS N500 is equipped with a neon lamp (option) for spectral calibration.

Calibration is possible at any wavelength by one click in the control software.

More capabilities


  • microscope can be equipped with a heating or cooling stage, vacuum or high pressure cell
  • fiber optics probe for remote measurements

Data Acquisition and Data Analysis software

RAMOS N500 software “Nano SPO” with powerful analytical functionality is designed for hardware operating, data acquisition and data analysis.

  • 2D and 3D image creation
  • Autofocus control during mapping
  • Automatic background subtraction, cosmic ray removing, peak shift imaging, etc.
  • Support for external spectral databases
  • Data export to popular file formats
  • Intuitive user-friendly interface
  • Compatible with Windows XP, Vista, 7